To obtain recombinant strain with high ice nucleation activity，iceA gene were amplified by PCR from ice nucleation active bacteria Erwinia ananas 110 and cloned into vector pMD19–T which was transformed into E.coli DH5α. The recombinant clones were screened by single and double digestion before sequenced. From the positive recombinant strain，iceA gene was subcloned into prokaryotic expression vector pET–23a(+)，resulting in recombinant plasmid pET–23a(+)-ice which was transformed into E.coli BL21(DE3)pLysS and induced by IPTG. SDS-PAGE indicated that ice nucleation active protein was expressed as inclusion bodies with molecular weight of about 180 000. Ice nucleation activity test showed there was no difference in ice nucleation activity under –5, –4, –3, and –2 ℃ between recombinant E.coli BL21(DE3)pLysS and wild ice nucleation active bacteria Erwinia ananas 110.