Five WRI1–Like WRI1 cDNA samples were isolated from Brassica napus (Xiangyou No.15 cultivar) by RT–PCR. Among the five cDNAs, BnWRI1–1 is 1 248 bp, BnWRI1–2 and BnWRI1–3 are 1 254 bp, BnWRI1–4 and BnWRI1–5 are 1 242 bp in length. Cluster Analysis showed WRI1 genes belong to transcription factor AP2/ERF gene family, which clustered in the same group with AtWRI1(At3G54320) gene of Arabidopsis thaliana. Alignment of the coding regions of WRI1 genes indicated 40 single nucleotide polymorphic sites. A nucleotide transition from A to G at position 892 in the coding sequence of BnWRI1–1，BnWRI1–2, BnWRI1–3 and at position 880 in the coding sequence of BnWRI1–4, BnWRI1–5 produced a specific restriction endonuclease cleavage site which was original identified by Bcl I. Further digestion test showed that Bcl I cleavage could differentiate the genome origin of Brassica napus At WRII–like WRII.