Abstract: Wolbachia are maternally inherited endosymbiotic bacteria, and they can manipulate the reproduction of their hosts. In this paper, we amplified the wsp gene fragment of Wolbachia in Andricus mukaigawae and Torymus sp. associated with the plant galls of A. mukaigawae, by using Wolbachia wsp-specific primers. Our results revealed that A. mukaigawae and Torymus sp. were respectively infected with Wolbachia strain Wa and Wb. We didn’t found the evidence for horizontal transmission between A. mukaigawae and Torymus sp. because of the difference of wsp sequence in Wolbachia strain Wa and Wb.