To verify the function of Lacc1 gene and investigate the relationship between the gene, promoter structure and transcription, expression for Ganoderma lucidum Laccase, we analyzed the amino acid structure of Lacc1 and upstream promoter region of putative laccase gene Lacc1, whilst studied the putative laccase gene transcription in Ganoderma lucidum strain P9 induced by Cu2+. Result of protein blast showed that Lacc1 had three Cu–oxidase conserved domains and multiple sequence alignments showed that Lacc1 had the highest homology with lcc3–3 of polyporus ciliatus, which was 71%, and the highly conserved laccase signature sequence L1–L4 was also found in Lacc1. The results of signal peptide prediction and sub–cellular location identification showed that Lacc1 is an extracellular (secreted) protein containing signal peptide. The prediction of upstream promoter region of gene Lacc1 showed 1 TATA box, 3 MRE (metal responsive element), 5 NIT2 (nitrogen regulatory element) and 1 STRE (stress responsive element). The expression of gene Lacc1 was up–regulated 2.8–fold, 4.9–fold, 1.6–fold and 1.9–fold at 6th day, 8th day, 10th day and 14th day after induction by 150 μmol/L Cu2+, respectively, in comparison to the reference condition. Via the analysis of Lacc1 sequence and Cu2+ induced gene expression, the Cu2+ induced transcription of Lacc1 gene was closely related to regulation motifs such as the responding elements for metals in the promoter region.