The major chemical properties of 92 tobacco samples from 8 counties in tobacco growing regions of Baise, Guangxi province collected in 2009 and 2010 were analyzed and clustered. The results indicated that the chemistry of flue-cured tobacco in Baise was suitable and harmonious with high potassium and low chlorine content and high stability in contents of potassium, total nitrogen and total nitrogen to nicotine ratio. The chemistry of the upper leaf was more suitable than that of the middle leaf. The contents of total sugar and starch were much different between years but nicotine content was stable. The difference of chemical components of tobacco samples among the eight regions was significant except for total nitrogen. The leaves from Jingxi, Debao, Xilin, Tianlin, Longlin and Napo counties were clustered into one category based on chemical property clustering, which represents the main tobacco growing areas of Baise, in which the chemistry was more suitable and harmonious. The leaves from Leye and Lingyun were clustered into another category, in which the content of sugar was slightly higher but the contents of nicotine and volatile alkali were slightly lower.