Different times of wood vinegar dilution were used to test frost resistance of blueberry leaves on various low temperature stress. The ion leakage percentage, content of soluble sugar, soluble protein and proline, and the change of SOD activity were measured for this purpose. Compared with the contrast, the results showed that, 1) Treatment with 100 dilution times could slow down the ion leakage percentage at all test temperature (the largest decline was 24.92% at 0 ℃), increase soluble sugar content at－5,－10 ℃ (the biggest increase was 15.04% at－5 ℃), increase soluble protein content at－5, －10,－20 ℃ (the biggest increase was 125.87% at－20 ℃), increase proline content at－5, －10,－20 ℃(the biggest increase was 63.69% at－10 ℃), and increase SOD activity at 4, 0, －5, －10,－20 ℃(the biggest increase was 12.42% at－10 ℃); 2) Treatment with 200 dilution times could also slow down the ion leakage percentage at all test temperature (the largest decline was 15.65% at－5 ℃), increase soluble sugar content at－5,－10,－20 ℃ (the biggest increase was 15.58% at－20 ℃), increase proline content at 4, 0 ℃ (the biggest increase was 20.32% at 0 ℃), increase SOD activity at 4, 0,－5,－10 ℃ (the biggest increase was 17.37% at －5 ℃); 3) Treatment with 300 dilution times could slow down the ion leakage percentage at－5 ℃, increase soluble protein and proline content at 4, 0 ℃, increase SOD activity at 4, 0,－5, －10 ℃. Wood vinegar could improve the capability of blueberry on frost resistance. Treatment with 100 dilution times was a better scheme.