Abstract: Ninety-five assessions from the mini core collection of Yunnan rice and their advanced backcross lines, which were obtained using the 95 accessions as donor, the Diantun 502 as the recurrent parent of Indica rice and the Hexi35 as the recurrent parent of japonica rice, were tested for their genetic variation, and the content of 3 functional components, namely, total flavonoids (TF), γ-aminobutyric acid (GABA) and resistant starch (RS) in the brown rice were determined. The results show that (1) there are significant difference in the variation coefficients and the ranges of FL, GABA and RS contents, with RS showing the highest variation coefficients followed by GABA and TF. (2) the contents of 3 functional components in brown rice of the acceptor are low, which were increased in the corresponding advanced backcross lines, the content of total flavonoids in brown rice of the donor were significantly higher than those of the advanced backcross lines, the content of GABA in brown rice of japonica advanced backcross lines was higher than those of the corresponding donor.The content of RS in brown rice of the donor and its advanced backcross lines are bimodally distributed, with low genetic variation, indicating the the content of RS is controlled by genotype. Key words: rice; mini core collection; advanced backcross lines; total flavonoids; γ-aminobutyric acid; resistant starch; genetic variation; Yunnan