The effects of different dosages (210, 105, 52.5 mg/mL) of Fuzhuan tea on quantity of Escherichia coli, Enterococcus, Bifidobacterium spp. and Lactobacillus spp. and on intestinal physiology were investigated using mice diarrhea model caused by Senna leaf. The results showed that high and middle dosage of Fuzhuan tea could not only promote the reproduction of intestinal beneficial microbes, but also restrain harmful microbes, thus preventing gut microbe disorder caused by Senna leaf. Among these effects, the growth of Lactobacillus spp. was the most affected character by Fuzhuan tea. In prevention groups with high-, middle- and low-dose Fuzhuan tea, the growth of Lactobacillus spp. was promoted, whose logarithm of bacterial number increased 3.309 0, 3.237 3 and 2.690 5 respectively on the 7th day compared with the bacterial number before treatment, indicating a dose dependent increase. High-dose Fuzhuan tea in treatment group could promote the growth of Lactobacillus spp.. Comparing the quantity of Lactobacillus spp. on the 7th d, the logarithm of bacterial number increased by 3.541 9 on the 11th d, better than berberine treated group (logarithm of the numbers of Lactobacillus spp. only increased 1.466 9). Middle- and low-dose Fuzhuan tea in treatment group could decrease Enterococcus, the logarithm of the numbers of which decreased by 0.423 6, 0.231 5 respectively, which was contrary to berberine treated group in which Enterococcus was increased. In treatment group, logarithm of the numbers of Escherichia coli on the 11th day were increased by 0.471 0, 0.335 3 and 0.510 9 respectively by high-, middle- and low-dose Fuzhuan tea compared to the quantity of Escherichia coli on the 7th day, which showed small difference compared to berberine treated group in which logarithm of the numbers of Escherichia coli increased by 0.556 2. There was no difference in intestinal characteristics between Fuzhuan tea treated mice group and the CK.