Major pathogens were isolated and identified from the soil of Jiangyong in Hunan province with serious ginger disease and antagonistic fungus against these pathogens were screened from Trichoderma spp. The results show that two bacterial pathogens G1 and H2 were isolated, of which G1 displayed a stronger pathogenicity. Morphological and 16S rDNA sequence characteristics showed that G1 belonged to Ralstonia slanacarum and H2 belonged to Erwinia spp. The flat confrontation experiment showed that No.23 Trichoderma viride belonged to Trichoderma harzianum had the strongest antagonism to pathogens G1 and H2, which can be used in biological control of ginger disease.