Difference in disease resistance to Sphaceloma ampelinum and Plasmopara viticola among two wild grape species (Vitis adenoclada Hand.-Mazz. and Vitis davidii Foëx) and two cultivated varieties (Red Globe and Hutai-8) were compared. By investigating the disease resistance in natural stands, the difference in disease resistance among scions which were grafted by the wild grape species and other wild grape resources were analyzed. Results showed that Vitis adenoclada Hand.-Mazz. were highly resistant to both Sphaceloma ampelinum and Plasmopara viticola. Vitis davidii Foëx showed resistance to Sphaceloma ampelinum, but not to Plasmopara viticola. The resistance was significantly improved when Vitis davidii Foëx was took as rootstock to form scion-rootstock combinations. Thus, Vitis adenoclada Hand.-Mazz. and Vitis davidii Foëx could be both used as resources for breeds with powerful resistance to fungal disease and Vitis davidii Foëx could be used as a source of breeding material for rootstocks.