Based on polymerase chain reaction (PCR), a rapid and accurate identification method was established for detection of the import plant quarantine pest Opogona sacchari. Genomic DNA of the samples was used as template in PCR amplification, and the PCR products were analyzed by gel electrophoresis. By PCR amplification, the detection time for Opogona sacchari was shortened to less than 8 hours. The test limit for genomic DNA concentration was 1 ng/μL, and the concentration above 10 ng/μL was the optimal.