To obtain general primers for detection and cloning of ACO gene of plant petal, a pair of degenerate prmiers was designed based on the published sequences of ACO genes of dicotyledonous woody plants in Genbank, which were used to clone ACO genes from petals in 7 plants, namely, Matthiola incana, Dendranthema morifolium, Prunus majestica Koehhe., Jasminum nudiflorum, Rhododendron yunnanense Franch., Malus halliana, Camellia japonica. The results indicated that specific fragments of about 800 bp could be amplified from all of the 7 plants and the sequences have not been registered on Genbank database. The sequences were submitted to Genbank database and the accessing numbers were JX503067, JX503068, JX503069, JX503070, KC112390, KC112392 and KC112393. The 7 sequences show high sequence identities with ACO genes of other plants through NCBI blast analysis, the highest homology of nucleotide sequence existed between Prunus majestica Koehhe. ACO gene and Prunus persica Batsch. ACO gene, which is 98%, and the lowest homology of nucleotide sequence existed between Jasminum nudiflorum ACO gene and Actinidia chinensis ACO6, which is 88%. It was found that the average sequence identity through comparison between any two samples was 77% by multiple sequence alignments.