The subterranean stems of Houttuynia cordata Thunb were employed for the purpose of establishing the rapid propagation system using explants sterilization, adventitious bud differentiation and multiplication, rooting and transplanting approaches in the paper. Results from the research were as follows: ① The best sterilization approach was to sterilize explants using streptomycin and penicillin (0.5 mg/L) for 12 hours firstly, then using HgCl2 (0.1%) for 12 minutes and HgCl2 (0.1%) for 10 minutes respectively; ② The best condition for the germination and growth of adventitious buds was adding 0.5 mg/L NAA and 2.0 mg/L 6–BA to MS medium, while, if 0.5 mg/L NAA and 1.0 mg/L 6–BA were added to MS medium, the propagation of adventitious buds could be effectively promoted. ③ Adding 4~5 mg/L GA3 to MS medium could promote proliferation and growth of adventitious buds; ④ NAA had a good promotional effect on improve budding and rooting of Houttuynia cordata Thunb. The optimal transplanting medium was cultivated soil and vermiculite which could result in 99% survival rate and healthy growing regeneration seedlings.