To better understand the genetic basis of blast resistance in the wild rice species Tianjinyeshengdao (TY), F2 population from the cross between TY and the rice cultivar CO39 which is highly susceptible to blast was constructed and respectively inoculated with isolates CHL1743, 110–2 and 318–2 in the growth chamber. The results showed that the resistant and susceptible plants in F2 population following inoculation fitted the ratio of 3∶1, indicating the resistance to isolates CHL1743, 110–2 and 318–2 in TY was controlled by a single dominant gene which was named as Pi2–1. To preliminary determine the chromosomal location of blast resistance gene, bulked segregant analysis (BSA) and recessive-class analysis (RCA) were conducted. The results indicated that Pi2–1 was located near the centromeric region of rice chromosome 6 and linked to the markers AP5659–5 and RM7213, with a distance of 0.9 cM and 1.4 cM, respectively.