Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity and relationship of 21 accessions of Vitis davidii Foëx. From 84 pairs of primers, 7 pairs were selected out. Sixty-one clear bands were amplified using these 7 pairs of primers, of which 34 were stable morphological bands, accounting for 55.7% of total bands. Revealed by NTSYS software, the genetic similarities of 21 accessions ranged from 0.80 to 0.99. Cluster analysis showed that 21 accessions were classified into 3 cluster groups with genetic similarity of 0.92. UPGMA cluster analysis showed that there are 6 types of Vitis davidii Foёx in Hunan, and we successfully deduced the suspected parents (which were lost during 3-time relocations) of Nongda No.1 to No.9 which come from different regions of Vitis davidii Foёx seeds by colchicine treatment.