Shoot apex with excellent agronomic characters and disease–free planting materials, which were used as explants, were studied on different types of culture medium, and on the culture media treated by adding different species and concentrations of plant hormones after sterilization with different treatments for Cynara scolymus L. ’s multiplication and rooting of tissue culture, to establish tissue culture seedling’s propagation technology system. The results showed that the best method of sterilization was the second combination, that was, The Cynara scolymus L. ’s shoot apex were sterilized by alcohol(70%) with 10 seconds firstly, then were sterilized by HgCl2(0.1%) for 8 minutes, and it showed the least pollution-induced infection. EM was the most suitable medium used in culturing Cynara scolymus L. in vitro, 6–BA was more effective than KT and 2–ip in the induction of artichoke bud proliferation. Regarding the primary culture, it recorded the best values of growth when the modified medium containing 1 mg/L 6–BA+0.5 mg/L NAA was used, while the fascicular buds grew best when the modified medium containing 0.5 mg/L 6–BA was used for the secondary culture. At the same time, by optimizing the rooting medium containing 1/2 EM, 1.0 mg/L NAA and 1.0 mg/L β–CD, the rate of rooting reached to 99.3%. And these young shoots cultured using the methods were vigorous with dark green leaf, then the transplant survival rate was over 90%.