Cultivars K326, Yunyan87 and Guiyansihao were used to investigate the curing characteristics and suitable harvest maturity of fresh flue-cured tobacco leaves in Ankang tobacco growing areas. The results indicated that the water loss of K326 was slow，and the leaves were resistant to curing and but relative easily to cure. The water loss of Yunyan87 and Guiyansihao were quick, and the leaves with normal resistance to curing were relative difficult to cure. For Guiyansihao, the shrinkage of CM1 maturity was the largest. For K326 and Yunyan87, the shrinkages of CM2 maturity were the largest while the shrinkage of CM1 maturity was the lowest, but the difference did not reach statistical significance. The proportion of top-middle-grade tobacco leaves and average price for K326 were the highest with XM2, CM2 and BM2 maturity, and for Yunyan87 were XM1, CM3 and BM2 maturity. For K326, leaves with XM2, CM2 and BM2 maturity showed the balanced chemical components and the best smoking quality.