Mother ferment liquid for optimizing mixed cultivation of lactobacillus plantarum, lactobacillus brevis and zygosaccharomyces rouxii was first prepared in employing composite fermentation of lactic acid bacteria and yeast. The effect of various carbon and nitrogen sources, their ratio, temperature, pH, inoculation quantity and rate on the growth of the three bacteria in celery liquid were tested. The results showed that the optimal condition for the three bacteria growth were: original pH was 6.5, the ratio of lactose to yeast extract was 5∶1, the temperature was 30 centigrade, the propotion in volume among lactobacillus plantarum, lactobacillus brevis and yeast was 2∶2∶1, and 7 percent inoculum dose. In these condition, the number of total lactic acid bacteria and yeast could reach 1.78×108 cfu/mL and 1.22×108 cfu/mL, respectively.