The auxin binding protein (ABP1) is involved in the response to auxin signal to induce the elongation of plant cell. In order to understand the interaction between ABP1 and CesA1, two chimera expression vectors are constructed to label ABP1 with GFP and CesA1 with CFP respectively. Two recombinants are transformed into tobacco leaf for transient expression in the same epidermal cell via infiltration. Green and cyan florescent signals are observed in the same pavement cells of the infiltrated leaf region by confocal microscope analysis, indicating proteins ABP1 and CesA1 are accumulated and overlaying distributed in the cell membranes. But in the endomembranes, the green signals are strong while the cyan signals are week, demonstrating that it is ABP1 but not CesA1 which is also collectively distributed in the endomembranes system. Cyan florescent signals are observed in interdigitation area of the pavement cells, which indicates intensive distribution of CesA1 and more celluloses were synthesized in this area.