To investigate the effects of dosage of nitrogen (N) fertilizer at early stage (ES), middle stage (MS) and late stage (LS), of application time of the regulating fertilizer (RF) and of row space (RS) on the source-sink relationship of the conventional japonica rice variety Kengjiandao10, the field experiment with multiple factors quadratic rotational-regressive-orthogonal combination design was conducted in cold area of China. The results showed that the amount of N-fertilizer applied at early stage had significant positive correlation with the maximum leaf area index (LAImax) and with the spikelets per unit area; the application time of RF had negative correlation with fertile glume numbers per unit area; and the RS had a quadratic curve relationship with LAImax and with the fertile glume numbers per unit area. It can be concluded that fertile glume numbers could be improved by increasing the amount of N-fertilizer at early stage or by interaction between N-fertilizer application and other cultivation measures (N-fertilizer amount at ES being 140 kg/hm2, N-fertilizer amount at MS and LS being 0 kg/hm2, RF being applied at 6.7 leaf stage, RS being 12 cm), thus improve the rice yield.