LTR retrotransposons are the largest and most widely distributed transposable elements in rice, of which Ty3-gypsy class is more numerous. A group of transcript fragments were amplified by RT-PCR and sequenced from cDNA of rice variety Acuche which were treated with 5 different stresses, with degenerate primers based on conserved region of reverse transcriptase in Ty3-gypsy retrotransposons. The characteristics of Ty3-gypsy sequences activated in different stresses were analyzed. The results showed that all the 5 different stresses could activate Ty3-gypsy sequences; Sequences responded to different stresses were highly conserved homologs and distributed crossly, only few of them showed specificity to stress, suggesting that many Ty3-gypsy retrotransposons could response to different stresses.