Mensurated and analyzed the relationship among the tobacco stem pulling force and pull-out displacement, tobacco stem diameter, rate of soil moisture, soil compactness. The results indicated that the pulling force and displacement curves reveal that the tobacco stem pulling is a comprehensive effect pulling force rising stage and the pulling force decreased stage, when the soil moisture content was low(13.2%), the soil compactness was large(18.6 kg/cm2), the pull-out displacement was 14 cm, the maximum pullout force was 1 654 N, when the soil moisture content was high(21.8%), the soil compactness was small(11.54 kg/cm2), the pull-out displacement was 16 cm, the maximum pullout force was 1 628 N; the tobacco stem diameter and the pulling force between was positively related to the linear regression relation, correlation coefficient is 0.88, tobacco stem diameter in the range of 26.1–38.3 mm, the pullout force value between 551–1 840 N; the soil moisture was negatively correlated with the pulling force, the soil moisture content was greater, pulling force was small; the soil compactness was positively correlated with the pulling force, the soil compactness was greater, the pulling force was greater.