In this study, physiological characteristics of flag leaf and grain yield during grain filling were investigated under conventional cultivation (T0) and different ridge cultivations including narrow ridge with terrace (T1), wide ridge with terrace (T2) and wide ridge with bed (T3) for rice varieties Y Liangyou 1, Huanghuazhan and Shanyou 63. The results showed under T1 and T2 cultivation, flag leaf chlorophyll content of rice was high with slowed leaf aging and higher net photosynthetic rate, which was conducive to the improvement of the protective enzyme activity to maintain normal cell metabolic. Under T0 and T3 cultivation, rice flag leaf chlorophyll content was low with fast aging, and the enzyme activity decreased by 24%–44%, 28%–46%, respectively. It was showed that grain yield in T1 cultivation was the highest, followed by T2 and T3 cultivation, grain yield of T3 cultivation was slightly higher than that of the T0 cultivation, but the difference did not reach statistical significance.