The coding region of a cloned cDNA of Ramie auxin binding protein gene (BnABP1) was inserted into a plant expression vector pCAMBIA1300-GFP under the control of promoter CaMV35S to construct a green fluorescent protein (GFP) fusion expression vector. The recombinant plasmid named pCAMBIA1300-GFP-BnABP1 was transformed into Nicotiana tabacum WS38 via Agrobacterium tumefaciens mediated transformation and transgenic tobacco was obtained by screening on antibiotic media and by PCR identification. The transgenic tobacco callus was detected under fluorescence microscope and the strongest green fluorescence signals were appeared in plasma membrane and inner membrane system. The results showed that Ramie auxin binding protein ABP1 was mostly concentrated in the membrane system of cell.