Indoor bioassay was carried out to study the effects of crude extract of Rhizoma et Radix Notopterygii on reducing the phytotoxicity of acetochlor to the rice shoots, and on induction of GSTs of rice shoots. The results showed that under the of acetochlor concentration of 0.05 mg/L, the plant height restored to 77.9%, 95.1%, 96.1%, 96.2% of those in the control, and the plant weight restored to 80.9%, 98.8%, 94.0%, 98.4% of those in the control, when mass concentrations of extract from Rhizoma et Radix Notopterygii were 0.05, 0.125, 0.25 and 1.25 g/L, respectively. GSTs activity in the above-ground shoots of rice can be increased by the induction of extract from Rhizoma et Radix Notopterygii, when the concentrations of the extract were 0.15 g/L, 0.20 g/L and 0.25 g/L, the GSTs activity in the above-ground shoots was respectively 1.5- , 2.0- and 2.1-fold higher than that of the control.