Resveratrol was extracted from grapevine by ethanol reflux extraction, and the quadratic regression equation y=–6.186 39+0.181 78x1–0.012 371x2+0.122 93x3+0.000 273x1x2–0.000 545x1x3–0.000 164x2x3–0.001 178 x12 –0.000 042x22 – 0.002 998x32 (r= 0.936 1，P<0.01) was obtained by selecting the ethanol concentration (x1), time for refluxing extraction (x2) and ratio of solvent to raw material (x3) as independent variables and the extraction rate of resveratrol as dependent variables. Then the extraction process was optimized using central composite design and response surface method. The optimal conditions of extraction process were as follows: extracted 2 times, 129.5 min for each extraction, with 90.2% ethanol and solvent to raw material ratio of 8.8∶1.