蓖麻栽培品种的遗传多样性及蓖麻籽脂肪酸组分分析
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973前期(2011CB111514)


Phylogenetic relationships of cultivars (hybrids) of Ricinus communis and analysis of fatty acid components
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    摘要:

    收集20份蓖麻栽培品种,以湖南永州野生种为对照,利用RAPD标记和GCMS法分析亲缘关系,并测定蓖麻籽脂肪酸组分。结果显示:①21条有效引物RAPDPCR扩增获得的总谱带数为75条,多态性谱带数为45条,多态性谱带比例为54.27%,利用UPGMA类平均法对扩增出的谱带进行遗传聚类分析,当遗传距离为0.146 8时,21份材料能较好地聚类;②蓖麻籽油主要含蓖麻油酸、油酸、亚油酸和少量的棕榈酸、硬脂酸、亚麻酸;在蓖麻籽脂肪酸中,蓖麻油酸含量为72.82%~89.54%、油酸2.97%~8.54%,亚油酸3.47%~10.55%,棕榈酸、硬脂酸含量约为1 %,亚麻酸含量约为0.5%,品种间蓖麻油酸的含量差别较大。

    Abstract:

    Phylogenetic relationship and fatty acid components of 21 cultivars (hybrids) and 1 wild Yongzhou variety of Ricinus communis were investigated. The results showed that: ①twenty RAPD primers were picked out from 296 10-mer primers for RAPD analysis; and 73 bands were obtained by use of the 20 primers, among the bands, 42 had polymorphism, accounting for 57.7%; the result of cluster analysis by use of UPGMA method showed that 21 genotypes could be classified together with genetic distant value of 0.1468. ②The castor oil contained mainly ricinoleic acid, oleic acid and linoleic acid, the contents of which were 72.82%~89.54%, 2.97%~8.54%, 3.47%~10.55%, respectively; there was little palmitinic acid, stearinic acid and linolenic acid in castor oil; the respective content of the palmitinic acid and stearinic acid was about 1%, and the content of the linolenic acid was around 0.5%. The content of ricinoleic acid among different cultivars of Ricinus communis showed big differences.

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虢婷婷,邢超,刘春林,阮颖.蓖麻栽培品种的遗传多样性及蓖麻籽脂肪酸组分分析[J].湖南农业大学学报:自然科学版,2012,38(4):373-376.

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  • 收稿日期:2012-02-05
  • 最后修改日期:2012-04-20
  • 录用日期:2012-07-11
  • 在线发布日期: 2012-07-20
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