Variation of physio-ecological and cytological features in continuous cropping ramie soil for 810, 45, 1 years in Zhongzhu No. 1 as material was studied by pot experiments for 2 years.The results showed that the plant height, stem diameter, shoot and root dry weight difference are not obvious in different treatments in 2010; but in the 2011 year, the plant height of A, B and C treatments are respectively to 50.2 cm, 104.6 cm, 112.6 cm in the secondly cropping period, A treatment was significantly lower than B, C, the stem diameter also showed the same laws; the shoot and root dry weight, A treatment was basically decay; the SOD activity and MDA content of three deal were no obvious differences in the firstly cropping and secondly cropping period of ramie, during the thirdly cropping period, the SOD activity of A treatment was significantly lower than B, C, the content of MDA is higher than that of B, C deal. in the 2010 and 2011years culture experiment, the continuous cropping obstacle effect in 810 years soil was significantly more than that of other tested soil, the continuous cropping obstacle on ramie occurred at seedling stage of the secondly cropping, the thirdly cropping stage are the most serious, root rot and retarded growth of the plants at 40 cm. Cytomorphological studies indicated that cell density reduced, cell arrangement loosely in the transverse section of ramie root caused by continuous cropping, and consequently affected root growth, reduced the absorb ability of nutrients and water from soil, retarded growth of the ramie plants. The plasamembrane lipid peroxidation of the plants led to impaired structure and function of plant cells under continuous cropping, it was suggested that there was a more inharmonious sink-source relation of ramie at the stressful condition of continuous cropping for 810 years, the so-called continuous cropping obstacle effect.