Fifty-three OsDIR genes distributed on 8 different rice chromosomes were identified according to rice bioinformatics resource. Gene structural analyses showed that 32 OsDIRs, accounting for 60.4% of the total, are intron-less gene; conserved domain prediction demonstrated that each of the OsDIR genes contains at least one conserved dirigent domain and 10 conserved motifs with different sizes, among which each motif showed different frequencies in all members of OsDIR gene family, were predicted through motif analysis. Multiple alignment of protein sequences showed that all the conserved protein sequences were located in the conserved domain, and most of the proteins are stabilized hydrophobic proteins, which expressed in most of the organelles with the most of which abundantly expressed in the cell wall, according to OsDIR protein sequences functional prediction. The OsDIR genes were classified into five subgroups according to phylogenetic analyses based on conserved protein domain sequences. Additionally, the replication characteristics of domain fragments and genes indicated that the OsDIR genes origin from the common ancestor/gene.