Male parent of super hybrid rice 0293 (0293) was used to study the genetic transformation of super hybrid rice by Agrobacterium-mediated transferring of plant resistant gene snc1（suppressor of npr1–1, constitutive 1）into rice plant. The results showed that the NMB medium is the optimal medium for callus induction on mature embryo of 0293, the optimal concentration of plant growth substances 2, 4–D and 6–BA are 3.0 mg/L and 0.2 mg/L, respectively; the transferring capability of Agrobacterium in LB liquid medium is found to be the best, and higher rate of resistant callus is obtained by the vacuum transferring under 2 days of co-cultivation; maltose is the optimal carbon source for resistant callus differentiation, and the best differentiation medium is NMB containing 0.4 mg/L KT, 2.5 mg/L 6–BA, 0.5 mg/L NAA and 0.5 mg/L IAA. After optimized the conditions of Agrobacterium-mediated transferring, 6 transformants with the target snc1 gene was obtained in this paper. .