不同海拔高度的烤烟淀粉合成动态研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Dynamic metabolic analysis of tobacco starch biosynthesis at different altitudes
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    对云烟87在贵州毕节地区不同海拔高度、不同发育时期叶片的超微结构、淀粉含量、淀粉合成相关基因的表达进行研究。结果表明:移栽后35~50 d(移栽前期)高海拔地区烟叶淀粉含量稍高,颗粒结合型淀粉合成酶基因(GBSSI)和淀粉分支酶基因(SBE)的表达较强,但叶绿体淀粉粒超微结构无明显差异;移栽后65~95 d,淀粉含量急剧增加,叶绿体淀粉粒体积和数量均增加,GBSSI和SBE的表达也较强;移栽后95 d,高海拔地区烟叶叶绿体已充分降解,中海拔地区的开始降解,低海拔地区叶绿体仍保持完整;一定海拔范围内,在烟叶发育前期,海拔高度越高,淀粉积累越多,发育后期高海拔地区烟叶叶绿体降解充分,且GBSSI和SBE的表达减弱,说明高海拔导致淀粉含量减少。

    Abstract:

    In order to elucidate the influence of different altitudes to starch biosynthesis, we compared the ultrastructure of leaves and the starch contents as well as the expression of several key genes related to starch biosynthesis in Yun87 at different development stages. The results showed that on the 35 d and 50 d after transplanting, the starch content and the expression level of granule-bound starch synthase I(GBSSI)were little higher in the high altitude than those in the low altitude. But no obvious difference was found in the ultrastructure of leaves among different altitudes. From the 65 d to 95 d, starch contents showed sharp increase trend. Simultaneously, chloroplasts gradually changed with rapid enlarging and increasing of the accumulated starch granules. Correspondingly, during this period, the starch content and the expression level of GBSSI and starch branching enzyme(SBE)were higher in the low altitude than those in the high altitude. Especially, on the 95 d, chloroplasts had fully degraded, began to degrade and were still keep intact in high, middle and low altitude respectively. These results indicated that in early developing period, the higher the altitudes, the more the starch contents, but in the later developing period, it was quite the opposite.

    参考文献
    相似文献
    引证文献
引用本文

杨永霞,石冰瑾,崔红.不同海拔高度的烤烟淀粉合成动态研究[J].湖南农业大学学报:自然科学版,2012,38(1):22-26.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-11-14
  • 最后修改日期:2011-11-14
  • 录用日期:2011-12-05
  • 在线发布日期: 2012-02-02
  • 出版日期:
文章二维码