In order to determine the connection force between the rape stem conveniently and accurately, two fixtures were designed according to the diameter distribution of main stem, branch stem and pod connection stem of rape variety Xiangzayou1613. One of the fixtures is angle adjustable that used for determining the connection force between the main stem and the branches, another is slot fixture that used for determining the connection force between pod connection stem and branches of different orders. Finally the fixtures was used in SANSCMT6104 microcomputer control electronic universal testing machine, the results show that the two fixtures can better grip the testing rape parts and determine all parameters.