To investigate the effect of dietary lipid on the body and blood biochemical parameters of Carassius auratus Gibelio, five diets containing 4% of either fish oil, soybean oil, lard, peanut oil or mixed oil (fish oil∶soybean oil∶lard=3∶4∶3) were fed to five groups of fish (average initial weight(6.04±0.05) g) respectively for 60 d. The results showed that the hepatosomatic index (HSI) and visceral-somatic index in lard group were the highest with HSI significantly higher than that in fish oil group and with visceral-somatic index significantly higher than that in mixed oil group (P<0.05). There was on significant difference in condition factor among different groups (P>0.05). The contents of total protein (TP) and albumin (ALB) shared the same change pattern with those significantly higher in fish oil group than in lard group (P<0.05) and with no significant difference among other groups (P>0.05). The activity change of glutamic oxaloacetic transaminase (AST) was similar to glutamic pyruvic transaminase (ALT), which were both significantly higher in lard group than in fish oil group (P<0.05) and no significant difference was observed among other groups (P>0.05). The concentration of triglyceride (TG) in fish oil group was the lowest followed by that in the soybean oil group, which were both significantly lower than that in mixed oil group (P<0.05). The concentration of the high density lipoprotein-cholesterol (HDL–C) in fish oil group was the highest followed by peanut oil, which were both significantly higher than that in lard group (P<0.05). As for GLB, total cholesterol (TC), blood glucose (BGLU), low density lipoprotein-cholesterol (LDL–C), alkaline phosphatase (AKP), cortisol, insulin (Ins) and glucagons (Glu), there was no significant change among five groups (P>0.05). These results explicated that the fish oil was the best suitable lipid source followed by soybean oil, peanut oil and mixed oil, while lard was the least suitable.