Four factors including the moisture content, seedling height, tobacco planting depth and transplanting speed, which influence the standing angles of seedlings transplanted by type 2ZY–1 tobacco transplanting machine, were analyzed by single factor and orthogonal tests. The result shows that planting depth exhibits the greatest impact on seeding standing, followed by soil moisture content, transplanting speed and the seedling height. Orthogonal test shows that under the moisture content of 30%, planting depth of 10 to 12 cm, seedlings height of 20 cm and transplanting speed of 1.0 m/s, the highest rate of seedlings with good standing angles is obtained.