Field plot trial was conducted to study the effect of combination of different organic fertilizer, namely pig manure, manure composting or biogas manure and chemical fertilizer on yield, quality and metabolism of carbon and nitrogen of spring maize. Results showed that all the combinations of organic manure and chemical fertilizer improved the photosynthesis characteristics and enzyme activities of nitrate reductase (NR) and sucrose phosphate synthase (SPS) in functional leaves, and the yields and quality of spring maize. Chemical fertilizer with 20% manure composting showed the best effect, with which the net photosynthetic rate and transpiration rate increased by 13.35% and 9.81%, respectively, the enzyme activities of NR and SPS in functional leaves of spring maize at filling stage increased by 17.96% and 9.98%, respectively, the contents of crude protein and raw starch in spring maize corn improved by 30.4% and 7.48%, respectively, nitrate contents decreased by 49.97% and grain yield increased by 7.94%, compared to those with pure fertilizer application.