Abstract: The karyotype of the progeny of a wide cross between Triticum dicoccoides and diploid wild oat was analyzed. The result showed that the karyotype formula of the hybrid progeny line 084 was 2n=6x=42=36m (4SAT)+6sm. The karyotype belonged to type 1A, which is an original and extremely symmetrical type. The evolution index of the Triticum dicoccoides, the diploid wild oat and the line 084 were 1, 4 and 1, respectively, indicating the degree of the evolution of the line 084 was consistent with the Triticum dicoccoides, and lower than the diploid wild oat. The chromosome length ratio of the line 084 was larger than the parents. And the average arm ratio, the asymmetry ratio, and the quantity ratio of the chromosomes with arm ratio >1.7 were all between corresponding ratios of Triticum dicoccoides and the diploid wild oat, suggesting that wide cross had certain significance in accelerating wheat evolution. The chromosome arm ratios between the progeny and the Triticum dicoccoides were very similar, and the lengths and arm ratios of four pairs of chromosomes of the line 084 were very similar to those in the diploid wild oat, indicating that the line 084 was true intergeneric hybrid.