A copper (Ⅱ) complex with nicotinic acid and Lαalanine was synthesized by the method of room temperature solid phase synthesis. The complex was characterized through elemental analysis, infrared spectra, ultraviolet spectra and Xray powder diffraction. The results showed that the COO group of nicotinic acid and the amino nitrogen and carboxylic oxygen of Lαalanine coordinated with Cu2+. Antibacterial test displayed that the complex have a certain antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Bacillus aeruginosus, Aeromonas hydrophila and Yersinia intermedia, and the activity against Bacillus subtilis was the strongest with the inhibition diameter of 13.4 mm at the concentration of 0.5 mmol/L. The minimal inhibitory concentration of the complex against bacillus subtilis was 0.031 25 mmol/L.