In order to reduce the loss rate and impurity rate of rapeseed during the mechanical harvesting process, Xiangzayou 1613, developed by Oilseed Crop Research Institute of Hunan Agricultural University, was used to measure the connection stress between the main stem and the branches，the stem and the pods 10 d before harvest, and the effects of growth time and position on the stress. The results showed that the connection stress between the main stem and branches ranged from 4.154 N/mm2 to 9.156 N/mm2, between the main stem and the pods ranged from 6.747 N/mm2 to15.821N/mm2, and between the pod and branches of different orders ranged from 4.842 N/mm2 to 15.400 N/mm2. On the main stem, the connection stress between the pods and the first and second branches displayed a marked decline along the plant growing direction, but the decline between the pods and the third branch was not obvious. In the plant growing direction (the abscissa axis), the connection stress between the main stem and the pods declined significantly. In the mature period, there was no change in the connection stress between the pods and the main stem or the branches of different orders.