The concentrations of Cu, Zn, Cd and Pb of NPK compound fertilizer (10 samples) and chick manure (21 samples) were measured for heavy metals accumulation by application of chemical fertilizer and manure in vegetable greenhouse soil in Shouguang. The results showed that contents of Cu, Zn, Cd and Pb in NPK compound fertilizer were lower than that in chicken manure, while the content of Cd in chicken manure was under the limitation (GB18877 2002). Pb content was low in both the compound fertilizer and the chicken manure. The inputs of Cu, Zn, Cd, and Pb to the greenhouse vegetable soil through fertilization every year in Shouguang were 3 516.282, 23 731.500, 19.473 and 69.011 g /ha respectively, and chicken manure accounted for 79%97% of the total fertilizer applied.