The rice MAPK gene BWMK1 is transcriptionally activated by fungal pathogen infection and wounding and acts as a positive regulator in plant disease resistance signaling. To further understand the BWMK1-mediated molecular signaling regulating pathways, a yeast two-hybrid system using BWMK1 as a bait was used to screen BWMK1 interacting proteins, and 7 BWMK1 interacting protein encoding genes BWIP1BWIP7 were isolated, BWIP1BWIP7 were located on 12, 2, 3, 10, 6, 4, 5 chromosomes, respectively. Homologous coding proteins were found for each isolated gene except BWIP2 and BWIP7, which indicated that BWIP1, 3, 4, 5 and 6 respectively coded for 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, myo-inositol phosphate synthease, putative phosphate transporter, WD-40 repeat containing protein and N-acetyl glutamate kinase 2.