ERF1(ethylene response factor 1) is a intranuclear transcription factor involved in the end of the ethylene signal transduction pathway. The sense and anti-sense cDNA sequences of CsERF1 were amplified from Citrus by RT–PCR and cloned into pMD–19T respectively, resulting in plasmids containing sense and anti-sense fragment of CsERF1 respectively. Plasmid containing sense CsERF1 and pFGC5941 were digested by XhoI and AscI, and the sense fragment of CsERF1 was inserted into binary vector pFGC5941 forming +CsERF1–pFGC5941, then the anti-sense fragment of CsERF1 was subsequently cloned into +CsERF1–pFGC5941 by SmaI and XbaI digestion and ligation of +CsERF1–pFGC5941 and plasmid containing anti-sense CsERF1, resulting in RNAi vector of CsERF1 with sense and antisense fragment flanking the intron of chalcone synthase a gene. Verified by PCR testing and sequencing, the RNAi vector of CsERF1 was successfully constructed. Antibiotic resistant buds were regenerated via Agrobacterium-mediated transformation of citrus explants which provides a way towards the elucidation of the function of CsERF1.