The simulation test was conducted to investigate nitrogen and phosphorus release status of non-point source pollution with the continuous application of animal manure. The results showed that the greater the application of animal manure the larger the release amount of N and P both in seepage and overlying water. The release of TN and TP in seepage water was always higher than in overlying water. In overlying water, the largest TN, TP emission was caused by soil processed with lower dairy manure, with the release amount per unit weight manure being 0.307 mg/g and 0.012 mg/g respectively. In seepage water, the largest TN emission was caused by soil sample processed with lower chicken manure, with the release amount per unit weight manure being 0.946 mg/g, and the largest TP emission was caused by soil sample processed with lower dairy manure, with the release amount per unit weight manure being 0.156 mg/g. The release amount of N, P per unit was bigger from the sample applied small amount of animal manure. The highest release rate of N appeared in pig manure followed by dairy and chicken manure. The dairy manure showed the highest release rate of P, while chicken manure showed the lowest. The three manure had the same feature that the lower application the stronger the capacity to release N and P.