Yewei B, a new rice germplasm was bred through transformed with genomic DNA of Oryza minuta into V20B by earstem injecting. Compared with V20B, the effective panicles numbers and the spikelets number per panicles of Yewei B are smaller, but the filled grains per spike are higher. The average seed setting percentage of Yewei B is found to be 11.8 % higher than that of V20B, but 1 000-grain weight of Yewei B is 6 gram less than that of V20B. The content of soluble sugar in leaf sheath of the top1st and top 2nd leaf decreases first then increases slightly and then decreases again in Yewei B. In V20B, the content of soluble sugar is less distinctly changed in leaf sheath of the top1st leaf, and it increases first then decreases and then increases again in leaf sheath of the top 2nd leaf. The brown rice percentage and head rice rate of Yewei B is similar to V20B, but the percentage of chalky grain and the area of chalkiness are 47.5% and 62.1 % lower than that of V20B respectively. Gel consistency of Yewei B is higher and the content of amylose is 39.0% lower than that of V20B.