Abstract：The 33 pairs of simple sequence repeats (SSR) primers were used to analyze the genetic polymorphism and genetic relationship of 84 tea cultivars. Totally 94 alleles were amplified using 33 SSR primers, the number of alleles per primer ranged from 2 to 4, averaged 2.85. The polymorphism information content (PIC) varied from 0.20 to 0.79, averaged 0.56. The observed heterozygosity (Ho) ranged from 0.08 to 0.97 and the expected heterozygosity varied from 0.14 to 0.86, averaged 0.53 and 0.62, respectively. The Shannon index of all accessions is 1.17. All of the results showed that the genetic polymorphism of all accessions were relatively abundant. The 84 accessions were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.54.