As a key member of Hox gene family, the Homeobox A10 gene (HoxA10) is an important candidate gene influencing reproduction traits in pigs, which plays important roles in embryonic development and cell differentiation. In this paper, HoxA10 gene was cloned from a Landrace pig by RT-PCR, and different tissues from the pig were tested by real-time PCR to determine the tissue-specific expression pattern of HoxA10. Results showed that the cloned HoxA10 cDNA of pig was 538 bp long, and it contained an open reading frame (ORF) of 285 bp encoding a peptide of 94 amino acid residues which showed 98.9% and 97.9% sequence identity to that of human and mouse respectively. In all tested pig tissues, HoxA10 expressed predominantly in forward leg muscle, followed by kidney, uterus, back leg muscle, oviduct, large intestine and abdominal fat. And little or no Expression of HoxA10 was detected in hypothalamus, cerebrum, cerebellum, thalamus, ovary, lung, stomach, small intestine, dorsal muscles and back fat. Key words: pig; HoxA10 gene; cDNA cloning; tissue expression