The physical and chemical properties, transmembrane region, hydrophobicity/hydrophilicity, secondary structure, functional domain, functional category and homology tree of Ptcor8 were analyzed by using bioinformatics methods .Results showed that Ptcor8 belongs to cold acclimation protein WCOR413 family and possesses a potential ORF of 621 bp sizes encoding 206 amino acid residues. Predicted by bioinformatic analysis tools such as ProParam, PSORT, MotifScan, CCD, TMHMM and NetPhos, the protein coded by Ptcor8, with molecular weight 2.28×106 and pI 9.4, possesses five hydropathicity regions, five transmembrane helix, six serine phosphorylation sites and one WCOR413 domain. Both subcell identification and molecular evolutionary investigation demonstrate that Ptcor8 protein belongs to PM type. Ptcor8 is a cold-induced gene and play an important role in imporving plant cold tolerance.