The CBF1 gene(CRT/DRE binding factor 1) was obtained from Arabidopsis thaliana by PCR and cloned into pMD18-T. Then CBF1 gene was sub-cloned into pBI121 vector to abtain the middle recombinant plasmid pBI121-CBF1 via XbaⅠand Sac I restriction enzyme sites. After that, the fragments containing 35S-CBF1 were digested from recombinant plasmid pBI121-CBF1 by EcoR I and Hind III, and directionally linked to the plant expression vector pCAMBIA1301 to get the plant expression vector p1301-CBF1. We transformed the embryogenic cell suspensions of wild banana(Musa intinerans Cheesm) with p1301-CBF1 via Agrobacterium-mediated transformation. Results from histochemical GUS assay and PCR suggested that CBF1 gene has been transferred into the wild banana.