This paper is an attempt to discuss the effect of Pb stress(0, 300, 500 mg/kg)on the growth and quality of K326 and Honghuadajinyuan (Hongda) flue-cured tobacco. Two species of flue-cured tobacco K326 and Hongda were selected to compare the tobacco agronomic traits, physiological, differences in quality indicators and content of Pb in plants and distribution in different soil with different Pb concentration in cultivation experiments. The result showed that the K326 was in the lower concentration of Pb, keeping a high level of agronomic traits, nitrate reductase and root vigor but absorbing less lead while high concentration of the Pb indicators of agronomic and quality indicators significantly reduced. The Hongda indexes of agronomic traits, nitrate reductase and root vigor less were affected by the Pb stress, showing strong tolerance with Pb. Its adaptability to Pb stress was better than that of the K326, however it also had strong absorption of Pb.