A strain of -galactosidase producing bacterium was screened out from the soil rich in soybean wastes of Western Hunan. The bacterium morphology observation, molecular biological identification and enzymatic characteristics was analysed. The bacterium was a stained gram positive and its 16S rDNA had homology with Lactoccus. It was identified as a strain bacterium of Lactoccus family. The bacteria produced an excretive α-galactosidase. The maximal α-galactosidase activity of shaking cultured supernatant was 6.21 U/mL. The -galactosidase showed the enzyme activity was the biggest with the value of pH5.5 and the tempertured of 45 ℃. The enzyme gene must be regulated in the typical glycolytic operon modle that the enzyme production could be induced by the substrate and inhibited by glucose. The best induction effect was obtained when the D-raffinose concentration reached 30 mol/mL and it was inhibited when glucose concentration reached 30 mol/mL.