With the extract obtained from radix peucedani by SFE(supercritical fluid extraction), coumarins were primarily purifed through silica-gel chromatography with solvent system of petroleum ether:ethyl acetate and further purification was processed by HSCCC(high speed counter current chromatography) with solvent system of petroleum ether:ethyl acetate:methanol:water(5∶5∶5∶4) at the flow rate of 2.0 mL/min under rotator speed of 850 r/min. Fractions were collected manually according to HSCCC chromatogram and then charaterized by NMR(nuclear magnetic resonance), which were peucedanocoumarin II, pteryxin, praeruptorin A and psoralen respectively.